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Acetyl-p53 (Lys139) mouse mAb

  • 服务简介

    Source/Purification:  

    This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys139 of human p53。 Antibodies are purified by protein G-conjugated agarose followed by

    acetylated p53 (Lys139) peptide affinity chromatography.


    Recommended Applications: 

    ELISA, Dot blot, Western blot.  Recommended antibody dilution: WB: 1:2000

    NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration。 Optimal dilutions/concentrations should be determined by the end user。 Not yet tested in other applications


                               

    Scientific Description:

     

    Acetyl-p53 (Lys139) mouse mAb

    Figure A: Dot blotting analysis on indicated amount of peptides using anti-p53K139ac mouse mAb. The list of peptides is included in the table on the right.

    Acetyl-p53 (Lys139) mouse mAb

    Figure B: Western blotting analysis on 30 μg of crude proteins from human MCF-7 whole cell lysates with (right) or without (left) treatment of etoposide (15ug/ml, 24 hours) and (500ng/ml, 24 hours)Trichostatin A using acetyl-p53 (Lys139) mouse mAb (1: 2000).

    Acetyl-p53 (Lys139) mouse mAb

    Figure C: Western blotting analysis on 30 μg of crude proteins from mouse brain and mouse liver whole tissue lysates using acetyl-p53 (Lys139) mouse mAb (1: 2000).


    Storage & Stability:

    The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.

  • 技术原理

    Cat#:

    PTM-185

    Ab Type:

    M

    Application:

    WB, Dot, ELISA

    Species:

    Human, Mouse, Rat, 

    Price: (100ul)

    3060

    Price: (20ul)

    700

  • 仪器设备

  • 技术优势

  • 应用领域

    ELISA, Dot blot, Western blot。 

    Recommended antibody dilution: WB: 1:2000



  • 参考文献

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